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Epizootic hemorrhagic disease virus 1
Epizootic hemorrhagic disease virus 1
規(guī)格:
貨期:
編號:B161707
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Epizootic hemorrhagic disease virus 1
商品貨號 B161707
Classification Reoviridae, Orbivirus, Epizootic hemorrhagic disease virus 1
Agent Epizootic hemorrhagic disease virus 1
Strain OV202
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images Micrograph of Epizootic hemorrhagic disease virus 1
Comments Strain fully sequenced by depositor.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
Vero E6 cells (ATCC® CRL-1586™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor J Loeb, University of Florida
Source Asymptomatic, farmed white-tailed deer, Gadsden County, Florida, USA. Isolated on September 22, 2015.
References

Subramaniam K, et al. Genomic Sequences of Epizootic Hemorrhagic Disease Viruses Isolated from Florida White-Tailed Deer. Genome Announc. 5(43): e01174-17, 2017. Pubmed: 29074661

Sun F, et al. Molecular typing of epizootic hemorrhagic disease virus serotypes by one-step multiplex RT-PCR. J Wildl Dis 50(3): 639-44, 2014. PubMed: 24807175

Cross References

Nucleotide (GenBank) : MF688826.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP1 protein (VP1) gene, complete cds

Nucleotide (GenBank) : MF688827.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP2 protein (VP2) gene, complete cds.

Nucleotide (GenBank) : MF688828.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP3 protein (VP3) gene, complete cds

Nucleotide (GenBank) : MF688829.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP4 protein (VP4) gene, complete cds

Nucleotide (GenBank) : MF688830.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP5 protein (VP5) gene, complete cds

Nucleotide (GenBank) : MF688831.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP6 protein (VP6) gene, complete cds

Nucleotide (GenBank) : MF688832.1 Epizootic hemorrhagic disease virus 1 isolate OV202 VP7 protein (VP7) gene, complete cds

Nucleotide (GenBank) : MF688833.1 Epizootic hemorrhagic disease virus 1 isolate OV202 NS1 protein (NS1) gene, complete cds

Nucleotide (GenBank) : MF688834.1 Epizootic hemorrhagic disease virus 1 isolate OV202 NS2 protein (NS2) gene, complete cds

Nucleotide (GenBank) : MF688835.1 Epizootic hemorrhagic disease virus 1 isolate OV202 NS3 protein (NS3) gene, complete cds

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