91精品国产综合成人,国产精品午夜自在在线精品,国内精品久久久久影院老司,国内精品久久久久久久久野战,国产精品美女久久福利网站,亚洲国产一成人久久精品,国产精品久久久久久一区二区三区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Transmissible gastroenteritis virus, porcine
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Transmissible gastroenteritis virus, porcine
Transmissible gastroenteritis virus, porcine
規(guī)格:
貨期:
編號:B162002
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Transmissible gastroenteritis virus, porcine
商品貨號 B162002
Classification Coronaviridae, Coronavirus
Agent Transmissible gastroenteritis virus, porcine
Strain Miller (TC-adapted)
Common Name Transmissible gastroenteritis virus, porcine
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
This item is derived by adaptation of virus isolated from small intestine of gnotobiotic infected pig (ATCC VR-743) to ST cells (ATCC CRL-1746) at ATCC.
Product Format frozen
Storage Conditions ?Store at -70°C or colder
Comments
This item is derived by adaptation of virus isolated from small intestine of gnotobiotic infected pig (ATCC VR-743) to ST cells (ATCC CRL-1746) at ATCC.
Recommended Host
Production Host:ST cells (ATCC CRL-1746)

Recommended Host: ?ST cells (ATCC? CRL-1746?)

Growth Conditions
Plant cells 24 hours in advance and infect when cultures are 90-100% confluent. Remove medium and rinse cells twice with VGM without TPCK-treated trypsin. Leave the second wash over the cells for 3 hours at 37.0°C in a humidified 5% CO2 atmosphere. Remove the second wash from the cells and then inoculate with a small volume of virus diluted with VGM supplemented with TPCK- treated trypsin (1 μg/ mL) to provide a MOI of about 0.1. Adsorb for 1 hour at 37.0°C in a humidified 5% CO2 atmosphere. End adsorption by adding VGM supplemented with 1 μg/ mL of TPCK- treated trypsin.
Incubate infected culture for 3 days at 37.0°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 70-80% of the culture.
Name of Depositor LJ Saif
Source
Small intestine of neonatal pig with porcine transmissible gastroenteritis.
References

Bohl EH, et al. Antibody responses in serum, colostrum, and milk of swine after infection or vaccination with transmissible gastroenteritis virus. Infect. Immun. 6: 289-301, 1972. PubMed: 4629259

Bohl EH, Kumagai T. The use of cell cultures for the study of TGE virus of swine. Proc. U.S. Livestock Sanit. Assoc. 69: 343-350, 1965.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
桂林市| 霍州市| 保德县| 新野县| 嵩明县| 师宗县| 公主岭市| 凌云县| 彭阳县| 黄大仙区| 香格里拉县| 郯城县| 桦甸市| 五原县| 昌吉市| 凉城县| 巢湖市| 孙吴县| 介休市| 娱乐| 灵丘县| 西和县| 德保县| 长宁县| 张家口市| 遂川县| 栾城县| 烟台市| 贡觉县| 双峰县| 临澧县| 溆浦县| 全州县| 云林县| 扶沟县| 山丹县| 于都县| 图片| 曲阜市| 青龙| 平果县|