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<em>Macaca mulatta</em> polyomavirus 1
<em>Macaca mulatta</em> polyomavirus 1
規(guī)格:
貨期:
編號(hào):B162813
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Macaca mulatta polyomavirus 1
商品貨號(hào) B162813
Classification Polyomaviridae, Betapolyomavirus
Deposited As Macaca mulatta polyomavirus 1
Agent Macaca mulatta polyomavirus 1
Strain Baylor [SVB2E (WT), SVB]
Common Name Simian virus 40, SV40
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images VR-1900 images
Comments C-terminal variable domain of T-antigen differs from that of SV40-776
Effect on Host
CPE, vacuolization, rounding, and sloughing
Recommended Host
CV-1 cells (ATCC® CCL-70™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 9-16 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor J Butel, Baylor College of Medicine
Source Houston, Texas, USA, 1961
References

Melnick JL, Stinebaugh S.  Excretion of vacuolating SV-40 virus (papova virus group) after ingestion as a contaminant of oral poliovaccine.  Proc Soc Exp Biol Med 109:965-968, 1962.  PubMed: 14472428

Stinebaugh S, Melnick JL.  Plaque formation by vacuolating virus, SV40. Virology 16: 348-349, 1962. PubMed: 13917252

Lanford RE, Butel JS.  Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen. Cell 37(3):801-813, 1984. PubMed: 6086146

Stewart AR, et al.  Identification of a variable region at the carboxy terminus of SV40 large T-antigen. Virology 221(2):355-361, 1996. PubMed: 8661447

Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047

Forsman ZH, et al.  Phylogenetic analysis of polyomavirus simian virus 40 from monkeys and humans reveals genetic variation.  J Virol 78(17): 9306-9316, 2004.  PubMed: 15308725

Sroller V, et al.  Influence of the viral regulatory region on tumor induction by simian virus 40 in hamsters. J Virol 82(2):871-879, 2008. PubMed: 17977966

Patel NC, et al.  Viral regulatory region effects on vertical transmission of polyomavirus SV40 in hamsters. Virology 386(1):94-101, 2009. PubMed: 19181358

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