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38-1
38-1
規(guī)格:
貨期:
編號(hào):B163721
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 38-1
商品貨號(hào) B163721
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a poorly conserved epitope on CB4 and neutralizes a restricted set of human CB4 isolates.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); neutralizing
Cellular Products
immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); neutralizing
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with coxsackievirus B4 (JVB strain, ATCC VR 184).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with a poorly conserved epitope on CB4 and neutralizes a restricted set of human CB4 isolates.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium (containing 1 mM sodium
pyruvate) with 4.5 g/L glucose, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2a; kappa light chain
Name of Depositor BS Prabhakar
Deposited As mouse (B cell); mouse (myeloma)
References

Prabhaker BS, et al. High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies. Nature 300: 374-376, 1982. PubMed: 6183593

Prabhaker BS, et al. Detection of conserved and nonconserved epitopes on Coxsackievirus B4: frequency of antigenic change. Virology 146: 302-306, 1985. PubMed: 2413617

Webb SR, et al. Neutralization epitope diversity of Coxsackievirus B4 isolates detected by monoclonal antibodies. J. Med. Virol. 20: 9-15, 1986. PubMed: 2428930

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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