91精品国产综合成人,国产精品午夜自在在线精品,国内精品久久久久影院老司,国内精品久久久久久久久野战,国产精品美女久久福利网站,亚洲国产一成人久久精品,国产精品久久久久久一区二区三区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 72A1
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
72A1
72A1
規(guī)格:
貨期:
編號:B163810
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 72A1
商品貨號 B163810
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications The antibody reacts with the 350000/220000 dalton viral envelope glycoprotein.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with disrupted fixed Epstein-Barr virus particles.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Epstein-Barr virus (EBV)
Cellular Products
immunoglobulin; monoclonal antibody; against Epstein-Barr virus (EBV)
Comments
The antibody reacts with the 350000/220000 dalton viral envelope glycoprotein.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype mouse IgG1
Name of Depositor GJ Hoffman
Deposited As mouse (B cell); mouse (myeloma)
References

Hoffman GJ, et al. Monoclonal antibody against a 250,000-dalton glycoprotein of Epstein-Barr virus identifies a membrane antigen and a neutralizing antigen. Proc. Natl. Acad. Sci. USA 77: 2979-2983, 1980. PubMed: 6248876

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
黔西县| 武定县| 乐亭县| 衢州市| 云浮市| 原阳县| 博客| 建始县| 东丰县| 巴塘县| 连平县| 武陟县| 江源县| 桃园市| 徐汇区| 梧州市| 井陉县| 沙坪坝区| 镇雄县| 柘荣县| 蓝田县| 阜城县| 九龙坡区| 建宁县| 西城区| 泾川县| 灵丘县| 鹰潭市| 卢氏县| 建始县| 双峰县| 韶关市| 綦江县| 厦门市| 宁都县| 岢岚县| 任丘市| 淮阳县| 葵青区| 鞍山市| 巧家县|