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A3.4H2
A3.4H2
規(guī)格:
貨期:
編號:B163873
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 A3.4H2
商品貨號 B163873
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain: BALB/c (B cell); BALB/c (myeloma)

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CTLA-4 (CD152)
Cellular Products
immunoglobulin; monoclonal antibody; against human CTLA-4 (CD152)
Comments
Animals were immunized with activated human T cell clones.
Spleen cells were fused with Sp2/0-Ag14 cells.
The antibody was able to block B7 ligand binding.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add media or fluid change as cell concentration increases. (Cells attach firmly to flask surface).
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a; kappa light chain
Name of Depositor VK Kuchroo, EA Greenfield
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Kuchroo VK, Greenfield EA. Specific antibodies and antibody fragments. US Patent 6,207,156 dated Mar 27 2001

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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