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produces protein estrogen receptor

Construct size (kb): 3.5

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pIC19R
Intact vector size: 2.695
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI EcoRV XbaI BglII XhoI SacI NruI HindIII PstI SalI
BamHI SmaI NarI
Polylinker sites: EcoRI ClaI EcoRV XbaI BglII XhoI SacI NruI HindIII PstI SalI
BamHI SmaI EcoRI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1
Cross references: DNA Seq. Acc.: L08911

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ESR
Genomic copy number: unique
Gene name: estrogen receptor
Contains complete coding sequence?: U
Chromosome: 6; Localization: 6 q25.1
Type of DNA: cDNA
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: M12674

Insert lengths(kb): 0.800000011920929

Gene product: estrogen receptor [ESR]

Alleles: A1, A2

Target Gene: estrogen receptor

Restriction digests of the clone give the following sizes (kb): BamHI--3.7; BglI--1.85, 1.2, 0.60; HindIII--3.7; PstI--3.3, 0.33; PvuII--2.4, 0.66, 0.50; EcoRI--2.7, 0.9.

The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.15; HindIII--0.69; PstI--0.04; PvuII--0.02; StuI--0.29, 0.39.

Overproduces the fusion protein in E. coli, the majority of which is found in inclusion bodies.

It binds DNA.

Contains the coding sequence for amino acids 109-374.

The recombinant fusion protein contains an additional 23 N-terminal amino acids and additional C-terminal amino acids.