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Distribution host: Escherichia coli DH1

Size (kb): 3.32

DESCRIPTION OF VECTOR:
Intact vector size: 3.320
Type of vector: plasmid
Cloning sites: PstI NarI BglII KpnI HindIII
Polylinker sites: NcoI PstI NarI EcoRV BglII KpnI HindIII
Construction: pACYC184, pARA12
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: p15A
MCS: HindIII...NcoI, ->
promoter: paraB, <-
coding sequence: araC, ->
marker(s): cmLR, <-

Vector: pAR3 (plasmid)

Promoters: Promoter paraB

Construction: pACYC184, pARA12

Marker(s):cmlR

Construct size (kb): 3.32

Features: marker(s): cmlR
promoter: paraB
replicon: p15A
MCS: HindIII...NcoI
coding sequence: araC

expression vector

Restriction digests of the clone give the following sizes (kb): BglII--3.4; EcoRV--2.5, 0.45 (doublet); PstI--3.4.

This vector is compatible with ColE1-derived plasmids and allows efficient expression of recombinant genes upon induction with arabinose.

These features make it convenient to be used in combination with standard gene expression vectors for the independently controlled production of two recombinant polypeptides in E. coli.

Temperature: 37.0°C