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lambdaEnk1
lambdaEnk1
規(guī)格:
貨期:
編號(hào):B229235
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 lambdaEnk1
商品貨號(hào) B229235
Designations lambdaEnk1
Species Homo sapiens, human
Vector
Construct size (kb): 47.0
Insert
DNA: genomic
Insert lengths(kb): 16.0
Tissue: fetal liver
Gene product: proenkephalin [PENK]
Alleles: A1, A2
Insert Size (kb) 16.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
EcoRI digest contains fragments of about 20, 11, 6.8, 3.2, 2.6, 1.23, 1.15 and 1.07 kb.
Restriction digests of the clone give the following sizes (kb): BamHI--29, 6.4, 5.6, 4.0, 1.6, 0.54; EcoRI--20, 11, 6.8, 3.2, 2.7, 1.3, 1.25, 1.1; HindIII--20, 5.8, 5.8, 4.25, 3.25, 2.7, 2.3, 1.9, 1.15; SalI--uncut; XbaI--20, 15, 12.
This genomic clone contains the entire coding region of proenkephalin.
To block repeated sequences in the probe, prehybridize with sonicated total human placental DNA instead of salmon sperm DNA. Final stringency wash with 0.1X SSC/0.1% SDS at 65-68C.
Enzyme(s) not detecting polymorphism: 29 enzymes including TaqI, MspI, RsaI, BamHI, PvuII, PstI.
References

Comb M, et al. Primary structure of the human proenkephalin gene. DNA 2: 213-229, 1983. PubMed: 6315326

Litt M, et al. Chromosomal localization of the human proenkephalin and prodynorphin genes. Am. J. Hum. Genet. 42: 327-334, 1988. PubMed: 2893547

Litt M, Buder A. A frequent RFLP identified by a human proenkephalin genomic clone (HGM9 symbol PENK). Nucleic Acids Res. 17: 465, 1989. PubMed: 2783483

James Douglass, personal communication

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