91精品国产综合成人,国产精品午夜自在在线精品,国内精品久久久久影院老司,国内精品久久久久久久久野战,国产精品美女久久福利网站,亚洲国产一成人久久精品,国产精品久久久久久一区二区三区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pPW121
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pPW121
pPW121
規(guī)格:
貨期:
編號:B234314
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pPW121
商品貨號 B234314
Designations pPW121
Depositors F Puta
Biosafety Level 1
Vector Information
Size (kb): 4.3860001564025880
Vector: pPW121 (plasmid)
Promoters: Promoter lambda PR
Construction: pUN121
Marker(s):ampR,tetR
Construct size (kb): 4.386000156402588
Features: marker(s): ampR
marker(s): tetR
operator: lac
promoter: lambda PR
replicon: pMB1
repressor gene: cI
Applications
vector permitting positive selection for inserts
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; HindIII--4.3; BamHI--4.3.
Postive selection cloning vector which confers tetR (recommended concentration 10 ug/ml) only when an insert has been successfully cloned into the cI repressor gene.
Plasmid was derived from pUN121 by introduction of a BglII site into the cI gene. Plasmid pUN121 was derived from pTR262 by addition of EcoRI and SmaI/XmaI sites in the cI gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Puta F, Wambutt R. Construction of a new Escherichia coli-Saccharomyces cerevisiae shuttle plasmid cloning vector allowing positive selection for cloned fragments. Folia Microbiol. 37: 193-198, 1992. PubMed: 1505881

Nilsson B, et al. An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis. Nucleic Acids Res. 11: 8019-8030, 1983. PubMed: 6316281

Frantisek Puta, personal communication

Shipped freeze-dried
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
霸州市| 垦利县| 宜昌市| 镇远县| 大化| 苏尼特右旗| 兰西县| 台北县| 惠安县| 砀山县| 内黄县| 浮梁县| 庆城县| 鱼台县| 仪陇县| 邓州市| 长宁区| 澜沧| 弥勒县| 湖南省| 呼伦贝尔市| 武乡县| 万安县| 张掖市| 泊头市| 固原市| 房山区| 梨树县| 彝良县| 镇坪县| 娱乐| 昭苏县| 高唐县| 浏阳市| 乐东| 淮滨县| 西乌| 林州市| 象山县| 苏尼特右旗| 阜新市|